Puffer Mines are Puffers that have been cybergenetically altered by Space Pirates to serve as guards. They only appear in Metroid Prime 3: Corruption's Pirate Homeworld. When Samus or any other creature makes contact with one, they violently rupture, releasing Phazon Gas into the air, often killing any creature in the immediate vicinity.
- "Avoid contact with the Puffer Mine. These creatures are cybergenetically enhanced version of the Puffers native to the Pirate Homeworld. Engineered by Space Pirates, they are now used as patrol animals. Their bodies have been filled with Phazon gas to be used as a defensive measure. If their shell is broken, their bodies explode and send a cloud of toxic gas out all around them. Nearby attackers rarely escape the volatile fumes." — Logbook "Puffer Mine" (Metroid Prime 3: Corruption)