Puddle Spores float on the surface of lava lakes, protected from potential predators by an impenetrable shell. When it does sense danger, a Puddle Spore will attempt to intimidate its opponent by opening its mantle cavity to increase its size. The mantle is vulnerable to weapons fire, however, and a direct hit to its mantle will cause a Puddle Spore to enter a defensive position in which it flips itself over and conceals itself in the magma. Its underside is flat and can be used as a platform to cross lava-filled areas.
A Puddle Spore can also slam its mantle shut, ejecting a harmful spread of projectile energy globules that will damage on contact.
- Considering their names and appearances, Puddle Spores may be an homage to Brinstar's plant creature, Spore Spawn.
- In unused text for the game, there is a mention of an Alpha Puddle Spore, an enhanced version of this creature originally set to appear in the Chozo Ruins.
- "Sentient floating lava mollusk protected by an impenetrable shell. A Puddle Spore opens when approached, attempting to intimidate with its size. When opened, direct fire to its mantle causes it to flip into a defensive position. If it can slam shut, it ejects a spread of harmful energy globules." — Logbook "Puddle Spore" (Metroid Prime)